Grimsby Borough Academy are the team that stand in the way of Town adding to last years Challenge Cup success.
Town have to pick themselves up after the disappointing draw away at Louth Town on Monday that saw them drop out of the title race.
Borough themselves also dropped out of the race for the league title on Wednesday night when they were defeated 4-0 by Skegness Town.
Kick off at Elsaforde Park is at 7:30 pm